VARIO one.

Membrane-Inlet Mass-Spectrometer (MIMS) technology for real-time measurements of all volatile components in liquids

The Vario one. provides users with an inexpensive access to MIMS technology. A user-friendly mass spectrometer that can be used to measure the concentrations of all volatile components from liquids and gases in real time and in continuous operation – without sample preparation.

Top 4 Advantages of Vario one.

Multi-substance analysis directly from liquids thanks to new inlet method with porous membrane

Vario one. is a membrane inlet mass spectrometer (MIMS) for measuring concentrations of all volatile substances directly from liquids. Due to the special inlet procedure, no sample preparation is required; this saves time and money. The unique feature of the Vario one. is its porous membrane. In contrast to dense solution-diffusion membranes, such as polydimethylsiloxane (PDMS) membranes, in multi-substance systems the dissolved volatile components are transferred into the gas phase according to their partial pressures.

The advantage for users is that the concentrations of over 30 different components can be measured simultaneously and without delay. This is of interest in reaction engineering when the educt and product concentrations in the system change rapidly. Measurement data is generated in milliseconds and the sensitivity of the measurement is in the ppb range. Due to the use of a quadrupole mass spectrometer (QMS), the Vario one. is very compact, robust and inexpensive.

Process control opportunities in biotechnology and chemistry

Advantages in Fermentation Processes

In the past, process mass spectrometers for fermentation processes could only measure from the gas phase (supply and exhaust air). The concentrations in the liquid where only determined through an estimate of the gas phase by means of physical equations, insofar as an equilibrium of volatile substances between liquid phase and gas phase could be established. Heavily volatile and non-volatile substances – such as carboxylic acids and fatty acids – could not be detected by gas mass spectrometers. The Vario one. offers a solution tailored for biotechnology. A membrane module is the preferred liquid inlet of the Vario one. With the help of a peristaltic pump integrated in the Vario one., a continuous sample flow (usually 1 to 10 milliliters per minute) is taken from a fermenter, passed through the membrane module and returned to the fermenter fully automatically (through a bypass system). Furthermore, the Vario one. is the first MIMS on the market that is designed for sterile use in biotechnology. The volatility of the substances in the sample stream can be increased, e.g. by tempering the membrane module.

Furthermore, the size of the reaction vessel is not important. The sample stream can be taken from 5 ml vessels or 10 m³ fermenters. Only 10 microliters per minute are transferred from the continuously flowing sample stream via the membrane into the gas phase. The loss of liquid is therefore very low.

Upscaling of biotechnological processes

The Vario one. offers the best prerequisites to accompany an upscaling of biotechnological processes from laboratory to production scale. In addition, the measured values can be passed on to a process control system, so that measured values from different sensors (e.g. NIR) can be combined for data evaluation. By using porous membranes, dissolved gases and larger molecules, such as alcohols, are simultaneously detected and quantified. Additional sensors such as oxygen and carbon dioxide (pO2, pCO2) sensors are not needed and the contamination risk for the fermentation process can be reduced. Furthermore, the data for the different substance concentrations are available in the same data quality and in real time, which facilitates data evaluation and better process control

Patented Membrane Inlet System

… enables simultaneous measurements of liquids and gases

The membrane module contains a chemically inert, organo- and hydrophobic porous membrane. At its interface, all volatile components in a liquid are continuously transferred through the membrane into a vacuum. From the gas flow in the vacuum, a small amount of molecules are led into a high vacuum and measured in this way. As a result, all volatile components in the liquid phase are continuously measured through the membrane. The technology is extremely reliable, has low operating costs and delivers faster and more comprehensive measurement results. (Patent: DE102015208250A1, EP3292563A1, date of disclosure 10.11.2016)

Technical Specifications


Measuring speed4 ms / amu
Measuring range100 ppb – 100 %
Mass ranges0,5 bis 100, 200, 300 and 512 amu possible
Weight~ 40kg
InterfacesCapillary inlet, optionally with operating temperatures up to 200 ° C
Ion sourceGas-tight cross beam electron impact ion source
ConnectionElectrical connection: 230 V, 50 Hz, 8 A; Power consumption <1.2 kVA
Calibration intervalAutomatic or manual
Sample intakeAutomated and continuous via integrated peristaltic pump
Mass filterHyperbolic quadrupole mass filter for better filter performance
Liquid / Gas consumptionµl/min
SoftwareIPI-QMS System- and User-Software

-System settings permanently stored in the hardware for reproducible measurement data

-Auto tuning for RF adjustment enables optimal and energy efficient operation

-User-friendly visualization between total ion current of the associated spectra and individual ion traces

-Export to third party software such as Chromstar 7 SCPA and OpenChrom

“In the future, computers may only weigh one and a half tons.“

– Assumption about the development of computers, 1949″


Thanks for your interest in Variolytics!

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Variolytics GmbH

  Nobelstraße 12, 70569 Stuttgart DE
  +49 176 1009 5817

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