Vario two. | Liquid- and Gasanalyzer

Analysis Allrounder for simultaneous analysis of substances from liquids and gases

The Vario two. is the big brother of the Vario one. Our Allrounder is the world’s first mass spectrometer that can measure simultaneously from the liquid and gas phase. The liquid and gas analyzer is especially used for fermentation monitoring and bioprocess engineering, but optimizes any process where parallel measurements from the liquid and gas phases are required. Simultaneous measurement from the liquid and gas dramatically increases the benefits of mass spectrometry relative to the initial cost. The combined liquid and gas measurement is patented by Variolytics and is not offered by any other supplier.

The Top 4 Advantages of the Analysis Allrounder

Analysis Allrounder for Liquid and Gas Measurements

The Vario two. with its patented inlet system is designed to measure simultaneously from the liquid and gas phases. The liquid and gas analyzer is the ideal combination of a classic quadrupole mass spectrometer for gas analysis and a membrane inlet mass spectrometer (MIMS technology) for liquid analysis. Since the Vario two. can be easily integrated into the industrial process, it measures continuously without disturbing the process. The instrument delivers consistently accurate measurement data with low measurement noise (Low Noise) and high linearity, allowing changes in reactions to be tracked in real time (from 25 milliseconds). The Allrounder adapts perfectly to your individual application. For example, the volatility of substances in the sample stream can be increased by changing the temperature control of the membrane module.

  • Fast

  • Precise

  • Low Noise

  • Flexible

User-Friendly Hardware and Software

The membrane inlets of the instrument allow online as well as inline measurements directly in the process, so that no sample preparation is necessary. This saves time and resources and allows to react quickly to changes in the process, thus optimizing it. The user-friendly software architecture simplifies real-time control of the instrument and visualizes the measurement data in the same user interface. The hardware is modular – so the membrane module can be replaced and, for example, autoclaved in a single step.

  • Process-oriented

  • User-friendly

  • Practical

Patented Inlet System

… enables simultaneous liquid and gas measurements

The membrane module contains a membrane at whose interface all volatile components in a liquid are continuously transferred through the membrane into a vacuum. From the gas flow in the vacuum, a small fraction of molecules is transferred to a high vacuum and measured in this way. (Patent: DE102015208250A1, EP3292563A1, disclosure date Nov. 10, 2016).

The Ideal Inlet for your Measurement Task

The Variolytics membrane inlets allow stable multi-parameter measurements from the liquid. The membrane module contains a hydro- and organophobic membrane. At the interface of the membrane, volatile components present in the liquid are continuously transferred through the membrane into a vacuum. Then these parameters are measured directly in the mass spectrometer. The membrane inlets are easily autoclavable and can be easily replaced if necessary.

Not sure which is the ideal inlet for your measurement task? Just contact us and we will be happy to advise you.

Flow Cell

With the flow cell, processes can be monitored online. For this purpose, a bypass is laid from the reaction site to the flow cell. For this reason, no probes or other components need to be inserted directly into the process. Thus, the process is not disturbed by the measurements. In addition, the module allows the measurement to be influenced purposefully for example, by setting a specific temperature or adding substances (e.g. acid to lower the pH).

In-Situ Sensor

With the in-situ sensor, measurements are made directly in the process, at the reaction site, and do not require a bypass. For this reason, the inlet has faster response times than the flow cell and thus there is no time delay in the measurement. Processes where very rapid changes occur can therefore be measured in real time. This membrane inlet is particularly suitable for measurements of dissolved gas concentrations.

Ideal for Multiplexing

… to measure from multiple sources simultaneously

Multiplexing allows customers to run multiple measurements on the same device. This allows different samples to be evaluated simultaneously. This leads to time savings in measurement tasks and reduces costs for expensive measurement technology. The Vario two. can also be easily integrated into a control system via our software. In biotechnology, two or even four bioreactors can thus be monitored and controlled simultaneously.

Latest Software Architecture

… for problem-free process integration

Tired of outdated software? So are we! The Vario two. offers a software architecture that can be easily integrated into process control systems or other hardware via common interfaces. A modern user interface and smart functions make it easy to use. Automated rotans even allow “stand-alone” operation for continuous measurements.

Technical Specifications

Measuring speed4 ms/amu
Measuring range100 ppb – 100%
Mass ranges0,5 up to 100, 200, 300 and
Dimensionsapprox. 434 x 526 x 519 mm (W x H x D)
Weightapprox. 60 kg
InterfacesCapillary inlet, optional with operating temperatures up to 200 °C
Ion sourceGas-tight cross beam electron impact ion source
Electrical connection230 V/AC, 50 Hz, 8 A; Power consumption < 1,2 kVA
Calibration intervalAutomatic or manual
Sample intakeAutomated and continuous via integrated peristaltic pump
Mass filterHyperbolic quadrupole mass filter for better filter perfomance
SoftwareIPI-QMS System- and User-software

  • System settings permanently stored in the hardware for reproducible measurement data
  • Auto tuning for RF adjustment enables optimal and energy efficient operation
  • User-friendly visualisation between total ion current of the associated spectra and individual ion traces
  • Export to thirt party software such as Chromstar 7 SCPA and OpenChrom


Thanks for your interest in Variolytics!

If you would like to get in touch with us, please fill out the contact form. We look forward to your message. Special inquiries or cooperation opportunities are also always welcome! Our team will get back to you as soon as possible.

Variolytics GmbH

  Meitnerstraße 6, 70563 Stuttgart DE
  +49 711 2525 9620



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